5JQ3
Crystal structure of Ebola glycoprotein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-09-12 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.07193 |
Spacegroup name | H 3 2 |
Unit cell lengths | 114.258, 114.258, 307.377 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 94.190 - 2.230 |
R-factor | 0.223 |
Rwork | 0.222 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3csy |
RMSD bond length | 0.007 |
RMSD bond angle | 1.244 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 94.190 | 2.290 |
High resolution limit [Å] | 2.230 | 2.230 |
Rmerge | 0.204 | |
Number of reflections | 38090 | |
<I/σ(I)> | 17.4 | 1.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 57.8 | 15.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 293 | 9% (w/v) PEG 6000 and 0.1 M Sodium citrate tribasic dihydrate |