5JPT

CRYSTAL STRUCTURE OF THE PRP43P DEAH-BOX RNA HELICASE IN COMPLEX WITH CDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0882
Spacegroup name	P 32 2 1
Unit cell lengths	118.070, 118.070, 252.150
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.309 - 2.935
R-factor	0.2061
Rwork	0.203
R-free	0.25740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xau
RMSD bond length	0.005
RMSD bond angle	0.974
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.310	3.040
High resolution limit [Å]	2.935	2.935
Rmerge	0.117	0.721
Number of reflections	44183
<I/σ(I)>	14.81
Completeness [%]	99.6
Redundancy	6.5
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	7.25% PEG 4000, 100 mM ammonium acetate and 50 mM sodium cacodylate pH 6.5