5JP5

Crystal structure of rat Galectin 5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2007-05-27
Detector	MAR CCD 130 mm
Wavelength(s)	0.95372
Spacegroup name	P 1 21 1
Unit cell lengths	66.088, 68.124, 95.346
Unit cell angles	90.00, 91.84, 90.00

Refinement procedure

Resolution	33.027 - 1.700
R-factor	0.1761
Rwork	0.174
R-free	0.21440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3nv1
RMSD bond length	0.006
RMSD bond angle	0.801
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.030	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.057	0.450
Number of reflections	92950
<I/σ(I)>	14.01	2.8
Completeness [%]	99.8	99.21
Redundancy	4.1	3.7
CC(1/2)	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	10% PEG 8000, 100 mM Tris pH 7.0, 200 mM MgCl2