5JP1
Structure of Xanthomonas campestris effector protein XopD bound to tomato SUMO
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-01-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9282 |
Spacegroup name | P 64 |
Unit cell lengths | 119.103, 119.103, 50.463 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 59.551 - 2.100 |
R-factor | 0.1832 |
Rwork | 0.181 |
R-free | 0.21680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2oiv 1tgz |
RMSD bond length | 0.002 |
RMSD bond angle | 0.507 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.550 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.089 | 0.465 |
Number of reflections | 23605 | |
<I/σ(I)> | 8.7 | 2 |
Completeness [%] | 98.1 | 99.4 |
Redundancy | 3.5 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M bicine (pH 9.0) 1.6M ammonium sulfate |