Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-24 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.909, 62.780, 94.030 |
| Unit cell angles | 90.00, 113.50, 90.00 |
Refinement procedure
| Resolution | 36.679 - 1.528 |
| R-factor | 0.1802 |
| Rwork | 0.179 |
| R-free | 0.20870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pfz |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.070 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless (0.3.11) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.410 | 1.550 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Rmerge | 0.065 | 0.609 |
| Number of reflections | 129149 | |
| <I/σ(I)> | 7.5 | 1.2 |
| Completeness [%] | 96.3 | 95.1 |
| Redundancy | 2.7 | 2.1 |
| CC(1/2) | 0.995 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.7 | 293 | 100 mM sodium citrate, 25% (w/v) PEG 3350 200 mM ammonium acetate |
