5JN5

Crystal structure of the D263Y missense variant of human PGM1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2015-10-25
Detector	RDI CMOS_8M
Wavelength(s)	1.00001
Spacegroup name	P 41 21 2
Unit cell lengths	172.988, 172.988, 100.001
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.704 - 1.750
R-factor	0.1814
Rwork	0.180
R-free	0.20880
RMSD bond length	0.008
RMSD bond angle	1.089
Data reduction software	XDS
Data scaling software	XDS
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.700	1.780
High resolution limit [Å]	1.750	1.750
Rmerge		1.587
Number of reflections	151916
<I/σ(I)>	15.9	1.5
Completeness [%]	100.0	100
Redundancy	14.4	13.3
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	LITHIUM OR AMMONIUM SULFATE (1.4 - 1.55 M) WITH 0.1 M TRIS, PH 7.5 OR MES PH 6.0