5JMR

X-ray structure of the furin inhibitory antibody Nb14

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.918409
Spacegroup name	I 41 2 2
Unit cell lengths	91.411, 91.411, 211.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.356 - 2.270
R-factor	0.1911
Rwork	0.190
R-free	0.21590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1op9
RMSD bond length	0.010
RMSD bond angle	1.154
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	35.356
High resolution limit [Å]	2.270
Number of reflections	21253
<I/σ(I)>	11.9
Completeness [%]	99.7
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293.15	10 mM Hepes, pH 7.5, 100 mM NaCl and 1mM CaCl2