5JMO
X-ray structure of furin in complex with the inhibitory antibody Nb14
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.976300 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 169.775, 50.039, 144.244 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 66.381 - 1.998 |
R-factor | 0.1647 |
Rwork | 0.163 |
R-free | 0.19710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4RYD (furin chain A or chain B) and 5JMR (antibody chain C or chain D) |
RMSD bond length | 0.007 |
RMSD bond angle | 1.054 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.381 | 2.120 |
High resolution limit [Å] | 1.998 | 1.998 |
Number of reflections | 83341 | |
<I/σ(I)> | 12.02 | |
Completeness [%] | 98.8 | |
Redundancy | 6.73 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | .1 M sodium acetate, pH 5.6, 16-18% PEG 3350 |