5JM1
Structure of tetrameric jacalin complexed with a trisaccharide, Gal alpha-(1,3) Gal beta-(1,4) Gal
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-08-28 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.490, 81.580, 63.000 |
| Unit cell angles | 90.00, 107.65, 90.00 |
Refinement procedure
| Resolution | 46.010 - 1.950 |
| R-factor | 0.18618 |
| Rwork | 0.180 |
| R-free | 0.23743 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ku8 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.897 |
| Data reduction software | iMOSFLM (1.0.5) |
| Data scaling software | SCALA |
| Phasing software | PHASER (2.3) |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.010 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.337 | |
| Number of reflections | 38922 | |
| <I/σ(I)> | 8.2 | 3.7 |
| Completeness [%] | 94.8 | 91 |
| Redundancy | 3.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.4 | 298 | 100 mM HEPES (pH 7.4), 15% poly(ethylene glycol) 8000, 10% (v/v) isopropanol |
