5JLH
Cryo-EM structure of a human cytoplasmic actomyosin complex at near-atomic resolution
EM specimen
| Vitrification | Yes |
| Instrument | GATAN CRYOPLUNGE 3 |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | FEI FALCON II (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.9 |
| Number of Particle | 138000 |
| Software | SPARX,iMODFIT,Coot,REFMAC,RELION,EPU,CTFFIND,MODELLER,UCSF Chimera |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
