5JL6
HUMAN PLACENTAL AROMATASE CYTOCHROME P450 (CYP19A1): ANDROSTENEDIONE COMPLEX #2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 140.314, 140.314, 118.403 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.900 - 3.000 |
| R-factor | 0.1962 |
| Rwork | 0.195 |
| R-free | 0.21692 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3s79 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.427 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.900 |
| High resolution limit [Å] | 3.000 |
| Rmerge | 0.088 |
| Number of reflections | 25528 |
| <I/σ(I)> | 25.8 |
| Completeness [%] | 98.2 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 277 | 20-30% PEG 4000, PHOSPHATE BUFFER, PH 7.4 |
