5JKL
Binary crystal structure of positively and negatively supercharged variants Ftn(pos) and Ftn(neg) from human heavy chain ferritin (Mg formate condition)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-15 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.00 |
Spacegroup name | P 4 |
Unit cell lengths | 126.620, 126.620, 174.860 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.850 - 1.800 |
R-factor | 0.1482 |
Rwork | 0.147 |
R-free | 0.17570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cei |
RMSD bond length | 0.020 |
RMSD bond angle | 1.811 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.5.6) |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.850 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.045 | 0.250 |
Number of reflections | 252223 | |
<I/σ(I)> | 22.9 | 5.16 |
Completeness [%] | 99.4 | 100 |
Redundancy | 4.46 | 4.41 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.19 M magnesium formate, 4 mg/mL Ftn(pos) and 4 mg/mL Ftn(neg) |