5JK9
Crystal structure of human IZUMO1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-20 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 64.936, 75.067, 108.885 |
| Unit cell angles | 77.65, 79.11, 70.66 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.19615 |
| Rwork | 0.194 |
| R-free | 0.23057 |
| Structure solution method | MIR |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.615 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.100 |
| Number of reflections | 106951 |
| <I/σ(I)> | 21.6 |
| Completeness [%] | 97.6 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 12-15% (w/v) PEG6000, 0.1 M sodium citrate pH 5.6 |
