5JK5
Phenylalanine hydroxylase from dictyostelium - BH2 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 6C1 |
Synchrotron site | PAL/PLS |
Beamline | 6C1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-05-26 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.968, 85.326, 74.894 |
Unit cell angles | 90.00, 110.23, 90.00 |
Refinement procedure
Resolution | 37.445 - 2.071 |
R-factor | 0.1677 |
Rwork | 0.165 |
R-free | 0.22760 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.026 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.070 |
Rmerge | 0.040 |
Number of reflections | 50582 |
<I/σ(I)> | 9.3 |
Completeness [%] | 99.1 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 26%(w/v) PEG 2000, 0.06 M PIPES |