5JJI
Rho transcription termination factor bound to rU7 and 6 ADP-BeF3 molecules
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-09 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.116 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 68.997, 198.567, 111.448 |
Unit cell angles | 90.00, 104.36, 90.00 |
Refinement procedure
Resolution | 47.425 - 2.601 |
R-factor | 0.2202 |
Rwork | 0.219 |
R-free | 0.24630 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.575 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER (2.1.4) |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.425 | 2.690 |
High resolution limit [Å] | 2.601 | 2.601 |
Rmerge | 0.081 | 0.630 |
Number of reflections | 88380 | |
<I/σ(I)> | 14 | 1.8 |
Completeness [%] | 99.7 | 99.1 |
Redundancy | 3.1 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7.5 | 291 | 200 mM KOAc, 40% MPD, 0.5% ethyl acetate |