5JII
Crystal structure of human IgG1-Fc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-03-02 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.8726 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.384, 75.088, 149.403 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.889 - 1.790 |
R-factor | 0.1828 |
Rwork | 0.181 |
R-free | 0.22060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1oqo |
RMSD bond length | 0.013 |
RMSD bond angle | 1.403 |
Data reduction software | XDS (VERSION January 10, 2014) |
Data scaling software | XDS |
Phasing software | PHENIX (1.10-2247) |
Refinement software | PHENIX (dev_1894) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.890 | 1.854 |
High resolution limit [Å] | 1.790 | 1.790 |
Rmerge | 0.047 | 0.930 |
Number of reflections | 52192 | |
<I/σ(I)> | 15.87 | 1.52 |
Completeness [%] | 97.8 | 95.97 |
Redundancy | 4.7 | 4.6 |
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 0.05 M Sodium phosphate, 16% (w/v) PEG 3350 |