5JIF
Crystal structure of mouse hepatitis virus strain DVIM Hemagglutinin-Esterase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2005-07-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.523, 88.819, 122.163 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.730 - 2.000 |
| R-factor | 0.20627 |
| Rwork | 0.205 |
| R-free | 0.22595 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MHV-S |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.468 |
| Data reduction software | EVAL15 |
| Data scaling software | EVAL15 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.410 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.096 | 1.184 |
| Number of reflections | 65139 | |
| <I/σ(I)> | 12.5 | 2.3 |
| Completeness [%] | 99.2 | 90.9 |
| Redundancy | 9.24 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 0.1M Tris-HCl pH 8.0, 0.05M NaF, 16% w/v PEG3350, 10% v/v glycerol |
