5JI1
Crystal Structure of GDF8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 29.606, 77.739, 119.544 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.965 - 2.250 |
| R-factor | 0.2377 |
| Rwork | 0.235 |
| R-free | 0.27730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hh2 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.951 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.23) |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.380 | 2.320 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.079 | 1.102 |
| Rmeas | 0.085 | |
| Rpim | 0.032 | |
| Total number of observations | 98443 | |
| Number of reflections | 13830 | |
| <I/σ(I)> | 18.2 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.1 | 7.3 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 100mM 2-(N-morpholino)ethanesulfonic acid pH 6.0, 40% 2-Methyl-2,4-pentanediol |
