5JHY

Crystal Structure of Fungal MagKatG2 at pH 5.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9729
Spacegroup name	P 21 21 21
Unit cell lengths	103.800, 109.720, 134.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.400
R-factor	0.15183
Rwork	0.151
R-free	0.17551
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ut2
RMSD bond length	0.027
RMSD bond angle	2.353
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.440
High resolution limit [Å]	1.400	1.400
Rmerge	0.082	0.440
Number of reflections	284103
<I/σ(I)>	10.6	2.8
Completeness [%]	94.9	97.8
Redundancy	3.9	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	277	15% PEG4000, 0.1 M sodium acetate, pH 4.6 (+ phosphate pH 5.5 soaking)