5JHH
Crystal structure of the ternary complex between the human RhoA, its inhibitor and the DH/PH domain of human ARHGEF11
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-05-15 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.541782 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 88.855, 119.049, 90.526 |
Unit cell angles | 90.00, 114.39, 90.00 |
Refinement procedure
Resolution | 82.440 - 2.300 |
R-factor | 0.1955 |
Rwork | 0.193 |
R-free | 0.23904 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.619 |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 82.440 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.071 | 0.513 |
Number of reflections | 73383 | |
<I/σ(I)> | 19.268 | 2.7 |
Completeness [%] | 96.3 | 93.2 |
Redundancy | 4.8 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 2% v/v Tacsimate pH 5.0, 0.1M Sodium citrate tribasic dihydrate pH 5.6, 16% PEG 3350 |