5JGM
tRNA guanine Transglycosylase (TGT) in co-crystallized complex with ethyl (2-(methylamino)-8-oxo-7,8-dihydro-1H-imidazo[4,5-g]quinazolin-6-yl)carbamate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-24 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.918409 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 91.182, 65.407, 71.064 |
Unit cell angles | 90.00, 96.64, 90.00 |
Refinement procedure
Resolution | 43.850 - 1.380 |
R-factor | 0.1353 |
Rwork | 0.134 |
R-free | 0.16160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1p0d |
RMSD bond length | 0.005 |
RMSD bond angle | 0.787 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1-2155_1492)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.290 | 1.460 |
High resolution limit [Å] | 1.380 | 1.380 |
Rmerge | 0.495 | |
Number of reflections | 84560 | |
<I/σ(I)> | 17 | 2.2 |
Completeness [%] | 98.9 | 97.4 |
Redundancy | 3.4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 288 | 13% PEG 8000,100mM MES, 1mM DTT, 10% DMSO |