5JGD
Crystal structure of human TAK1/TAB1 fusion protein in complex with ligand 12
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2009-12-14 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0000 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 58.167, 133.673, 141.111 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 97.040 - 3.101 |
R-factor | 0.183 |
Rwork | 0.181 |
R-free | 0.21480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2eva |
RMSD bond length | 0.008 |
RMSD bond angle | 1.237 |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 97.040 | 70.555 | 3.270 |
High resolution limit [Å] | 3.101 | 9.810 | 3.101 |
Rmerge | 0.042 | 0.498 | |
Number of reflections | 10345 | ||
<I/σ(I)> | 14.1 | 14.9 | 1.4 |
Completeness [%] | 100.0 | 99.5 | 100 |
Redundancy | 7.1 | 5.8 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | 1.7M sodium potassium phosphate, 20%(v/v) Glycerol as cryoprotectant |