5JG6
APC11-Ubv shows role of noncovalent RING-Ubiquitin interactions in processive multiubiquitination and Ubiquitin chain elongation by APC/C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 131.066, 35.193, 72.768 |
| Unit cell angles | 90.00, 120.42, 90.00 |
Refinement procedure
| Resolution | 36.326 - 2.001 |
| R-factor | 0.187 |
| Rwork | 0.185 |
| R-free | 0.21950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4R2Y and 1UBQ |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.947 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 36.326 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 19650 |
| <I/σ(I)> | 10.2 |
| Completeness [%] | 99.7 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M Ammonium acetate, 0.1 M Sodium acetate pH 4.6, 33% PEG4000 |
