5JFS
Crystal structure of TrkA in complex with PF-00593174
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-09 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1 |
Spacegroup name | P 64 |
Unit cell lengths | 125.220, 125.220, 46.510 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.300 - 2.070 |
R-factor | 0.171 |
Rwork | 0.170 |
R-free | 0.19090 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.030 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | BUSTER |
Refinement software | BUSTER (2.9.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 107.430 | 2.180 |
High resolution limit [Å] | 2.070 | 2.070 |
Number of reflections | 24621 | |
<I/σ(I)> | 12.3 | |
Completeness [%] | 98.6 | |
Redundancy | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 277 | protein was concentrated to 10mg/ml. Crystals of complexes were grown using the hanging drop vapour diffusion method from 0.1M HEPES pH7.5, 0.1M MgCl2 and 10% EtOH at 4C |