5JF1
Crystal structure of type 2 PDF from Streptococcus agalactiae in complex with actinonin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-23 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.840, 66.160, 88.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.410 - 2.000 |
| R-factor | 0.1536 |
| Rwork | 0.151 |
| R-free | 0.19805 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2aie |
| RMSD bond length | 0.031 |
| RMSD bond angle | 2.393 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.408 | |
| Number of reflections | 17013 | |
| <I/σ(I)> | 14.14 | 5.62 |
| Completeness [%] | 98.8 | 93.4 |
| Redundancy | 6.97 | 6.94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 9% PEG-8000, 100mM Na Cacodylate pH6.5, 400mM Zn acetate |
