5JF1
Crystal structure of type 2 PDF from Streptococcus agalactiae in complex with actinonin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-23 |
Detector | ADSC QUANTUM 4r |
Wavelength(s) | 0.934 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.840, 66.160, 88.860 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.410 - 2.000 |
R-factor | 0.1536 |
Rwork | 0.151 |
R-free | 0.19805 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2aie |
RMSD bond length | 0.031 |
RMSD bond angle | 2.393 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.110 |
High resolution limit [Å] | 1.990 | 1.990 |
Rmerge | 0.408 | |
Number of reflections | 17013 | |
<I/σ(I)> | 14.14 | 5.62 |
Completeness [%] | 98.8 | 93.4 |
Redundancy | 6.97 | 6.94 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 9% PEG-8000, 100mM Na Cacodylate pH6.5, 400mM Zn acetate |