5JER
Structure of Rotavirus NSP1 bound to IRF-3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2015-07-03 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.772, 107.924, 135.647 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.940 - 2.913 |
R-factor | 0.2133 |
Rwork | 0.210 |
R-free | 0.25030 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.589 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 47.940 |
High resolution limit [Å] | 2.900 |
Number of reflections | 22409 |
<I/σ(I)> | 15.7 |
Completeness [%] | 100.0 |
Redundancy | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M Bis-Tris pH 5.5, 200 mM MgCl2, 23% PEG 3350 |