5JEA

Structure of a cytoplasmic 11-subunit RNA exosome complex including Ski7, bound to RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97852
Spacegroup name	P 21 21 21
Unit cell lengths	106.080, 182.530, 250.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	66.688 - 2.650
R-factor	0.2174
Rwork	0.215
R-free	0.25860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ifd
RMSD bond length	0.004
RMSD bond angle	0.766
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.690	2.750
High resolution limit [Å]	2.650	2.650
Rmerge	0.211	1.762
Number of reflections	272923
<I/σ(I)>	7.33	1.14
Completeness [%]	100.0	99.99
Redundancy	10.26	8.16
CC(1/2)	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293.15	27 % 2-Methyl-2,4-pentanediol (MPD), 0.1 M 2-(N-morpholino)ethanesulfonic acid (MES) pH 6 and 10 mM CaCl2.