5JDS
Crystal structure of PD-L1 complexed with a nanobody at 1.7 Angstron resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9777 |
| Spacegroup name | P 61 |
| Unit cell lengths | 83.130, 83.130, 73.230 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.600 - 1.700 |
| R-factor | 0.17969 |
| Rwork | 0.178 |
| R-free | 0.20439 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.455 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 51.340 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 31640 |
| <I/σ(I)> | 9.6 |
| Completeness [%] | 99.9 |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 295 | 2M NaCl, 1.4M (NH4)2SO4 |
