5JD0

crystal structure of ARAP3 RhoGAP domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97931
Spacegroup name	P 43 21 2
Unit cell lengths	108.510, 108.510, 95.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.364 - 2.300
R-factor	0.2222
Rwork	0.220
R-free	0.25960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ee5
RMSD bond length	0.008
RMSD bond angle	0.916
Data reduction software	SCALA
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	76.728	38.364	2.420
High resolution limit [Å]	2.300	7.270	2.300
Rmerge		0.077	0.642
Rmeas	0.147	0.083	0.679
Rpim	0.049	0.029	0.219
Total number of observations	232624	7487	34522
Number of reflections	25608
<I/σ(I)>	11.1	19.7	3.8
Completeness [%]	99.1	98.6	100
Redundancy	9.1	8.1	9.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	293	100 mM HEPES, 10% PEG8000