5JCZ
Rab11 bound to MyoVa-GTD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-06-06 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.980110 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 215.790, 128.420, 89.020 |
Unit cell angles | 90.00, 98.27, 90.00 |
Refinement procedure
Resolution | 48.879 - 2.056 |
R-factor | 0.1811 |
Rwork | 0.180 |
R-free | 0.20830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lx1 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.593 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.880 | 2.180 |
High resolution limit [Å] | 2.056 | 2.056 |
Rmerge | 0.555 | |
Number of reflections | 146774 | |
<I/σ(I)> | 20.58 | |
Completeness [%] | 98.6 | |
Redundancy | 7.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 290 | 7% (w/v) PEG-8000, 50 mM Bicine, 50 mM Tris, 30mM sodium fluoride, 20% (v/v) ethylene glycol |