5J9W

Crystal structure of the NuA4 core complex

Experimental procedure

Experimental method	LAUE
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	173
Detector technology	CCD
Collection date	2015-12-30
Detector	ADSC QUANTUM 4r
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	87.260, 138.570, 167.868
Unit cell angles	90.00, 97.11, 90.00

Refinement procedure

Resolution	29.878 - 2.800
R-factor	0.228
Rwork	0.227
R-free	0.27110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.650
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.800
Number of reflections	80437
<I/σ(I)>	6.1
Completeness [%]	83.1
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		289	100 mM HEPES (pH 7.5), 9% PEG 20000, 8% glycerol, 7% 2-Propanol, 10% 1,6-Hexanediol, 10 mM DTT