5J9W
Crystal structure of the NuA4 core complex
Experimental procedure
Experimental method | LAUE |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 173 |
Detector technology | CCD |
Collection date | 2015-12-30 |
Detector | ADSC QUANTUM 4r |
Wavelength(s) | 0.987 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 87.260, 138.570, 167.868 |
Unit cell angles | 90.00, 97.11, 90.00 |
Refinement procedure
Resolution | 29.878 - 2.800 |
R-factor | 0.228 |
Rwork | 0.227 |
R-free | 0.27110 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.650 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.800 |
Number of reflections | 80437 |
<I/σ(I)> | 6.1 |
Completeness [%] | 83.1 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 289 | 100 mM HEPES (pH 7.5), 9% PEG 20000, 8% glycerol, 7% 2-Propanol, 10% 1,6-Hexanediol, 10 mM DTT |