5J7L
Structure of the 70S E coli ribosome with the U1052G mutation in the 16S rRNA bound to tetracycline
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 212.410, 435.700, 625.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.610 - 3.000 |
| R-factor | 0.172 |
| Rwork | 0.172 |
| R-free | 0.19300 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.870 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | BUSTER |
| Refinement software | BUSTER-TNT (2.11.6) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.610 |
| High resolution limit [Å] | 2.999 |
| <I/σ(I)> | 12.9 |
| Completeness [%] | 99.2 |
| Redundancy | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 291 | PEG 8k, Spermidine, Putrescine, Ammonium Chloride, Magnesium Chloride, MES pH 6.5 |
