5J6H
Recognition of the MHC class Ib molecule H2-Q10 by the natural killer cell receptor Ly49C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9436 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.420, 81.660, 97.920 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.470 - 2.300 |
R-factor | 0.1802 |
Rwork | 0.177 |
R-free | 0.24340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yn6 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.080 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 42.000 |
High resolution limit [Å] | 2.300 |
Rmerge | 0.241 |
Number of reflections | 20349 |
<I/σ(I)> | 6.6 |
Completeness [%] | 99.8 |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 8% Peg3350, 0.1M sodium malonate ph 5.0, 0.01M b-nicotinamide adenine dinucleotide hydrate |