5J60
Structure of a thioredoxin reductase from Gloeobacter violaceus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-22 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.00004 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.278, 122.042, 139.153 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.222 - 1.900 |
| R-factor | 0.1779 |
| Rwork | 0.177 |
| R-free | 0.20150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q7v |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.982 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2341: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.222 | 1.968 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.068 | 1.523 |
| Number of reflections | 112104 | |
| <I/σ(I)> | 24.02 | 1.59 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.3 | 12.3 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 30 & PEG-400 0.1 M Natrium Acetate, pH 4.5 0.2 M Calcium acetate |
