5J5Y
Translation initiation factor 4E in complex with m2(7,2'O)GppCCl2ppG mRNA 5' cap analog
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-04-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.918 |
Spacegroup name | P 1 |
Unit cell lengths | 38.040, 38.060, 146.740 |
Unit cell angles | 88.36, 95.63, 103.54 |
Refinement procedure
Resolution | 37.001 - 1.750 |
R-factor | 0.239 |
Rwork | 0.236 |
R-free | 0.28590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1l8b |
RMSD bond length | 0.007 |
RMSD bond angle | 0.951 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2152: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.790 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.052 | 0.430 |
Number of reflections | 72989 | |
<I/σ(I)> | 12.26 | |
Completeness [%] | 90.6 | |
Redundancy | 2.66 | |
CC(1/2) | 0.997 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 15% PEG 3350, 2% 1,4-dioxane, 0.1 M Tris |