5J4E
Crystal structures reveal signaling states of a short blue light photoreceptor protein PpSB1-LOV (Photoexcited state)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-08 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.96863 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.409, 71.080, 91.369 |
| Unit cell angles | 90.00, 91.02, 90.00 |
Refinement procedure
| Resolution | 45.677 - 2.670 |
| R-factor | 0.1898 |
| Rwork | 0.186 |
| R-free | 0.26080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5j3w |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.383 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 45.680 |
| High resolution limit [Å] | 2.670 |
| Rmerge | 0.048 |
| Number of reflections | 18700 |
| <I/σ(I)> | 17.9 |
| Completeness [%] | 99.5 |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | Na-cacodylate, MgCl2, PEG 3000 |
