5J3T
Crystal structure of S. pombe Dcp2:Dcp1:Edc1 mRNA decapping complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-19 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.809, 41.371, 93.718 |
| Unit cell angles | 90.00, 114.04, 90.00 |
Refinement procedure
| Resolution | 23.430 - 1.600 |
| R-factor | 0.1876 |
| Rwork | 0.186 |
| R-free | 0.21910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5j3y |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.100 | 1.680 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 45926 | |
| <I/σ(I)> | 18.9 | 1.6 |
| Completeness [%] | 99.1 | 96.4 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.8 | 292 | 1.55 M sodium formate, 0.1 M sodium acetate (pH 4.8) |
