5J3J
Crystal Structure of human DPP-IV in complex with HL1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-01-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97852 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.360, 126.820, 112.620 |
| Unit cell angles | 90.00, 99.95, 90.00 |
Refinement procedure
| Resolution | 55.110 - 2.750 |
| R-factor | 0.23813 |
| Rwork | 0.235 |
| R-free | 0.29803 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.952 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 57.400 |
| High resolution limit [Å] | 2.750 |
| Number of reflections | 44595 |
| <I/σ(I)> | 4.9 |
| Completeness [%] | 94.8 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8 | 289 | 100mM Tris pH 8, 18% PEG 3350, 200mM NaCl |
