5J3D

Crystal structure of human Fab 14N4 in complex with post-fusion RSV F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	80
Detector technology	CCD
Collection date	2015-12-11
Detector	RAYONIX MX-225
Wavelength(s)	0.97872
Spacegroup name	P 42 21 2
Unit cell lengths	235.130, 235.130, 220.142
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.500 - 4.077
R-factor	0.2579
Rwork	0.257
R-free	0.28190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rrr
RMSD bond length	0.009
RMSD bond angle	1.317
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.500	4.250
High resolution limit [Å]	4.077	4.077
Rmerge	0.296	1.191
Number of reflections	91490
<I/σ(I)>	5.5	1.7
Completeness [%]	98.1	98.7
Redundancy	8.3	8.5
CC(1/2)	0.987	0.511

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	2 M ammonium sulfate, 5% 2-propanol