5J3D
Crystal structure of human Fab 14N4 in complex with post-fusion RSV F
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2015-12-11 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.97872 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 235.130, 235.130, 220.142 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.500 - 4.077 |
R-factor | 0.2579 |
Rwork | 0.257 |
R-free | 0.28190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rrr |
RMSD bond length | 0.009 |
RMSD bond angle | 1.317 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.500 | 4.250 |
High resolution limit [Å] | 4.077 | 4.077 |
Rmerge | 0.296 | 1.191 |
Number of reflections | 91490 | |
<I/σ(I)> | 5.5 | 1.7 |
Completeness [%] | 98.1 | 98.7 |
Redundancy | 8.3 | 8.5 |
CC(1/2) | 0.987 | 0.511 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 2 M ammonium sulfate, 5% 2-propanol |