5J39
Crystal Structure of the extended TUDOR domain from TDRD2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-29 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97957 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.692, 53.798, 108.063 |
| Unit cell angles | 90.00, 84.26, 90.00 |
Refinement procedure
| Resolution | 34.110 - 1.950 |
| R-factor | 0.1922 |
| Rwork | 0.190 |
| R-free | 0.22220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fdr |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.23) |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (2.10.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 53.800 | 53.800 | 2.000 |
| High resolution limit [Å] | 1.950 | 8.940 | 1.950 |
| Rmerge | 0.087 | 0.020 | 1.114 |
| Rmeas | 0.101 | 0.023 | 1.296 |
| Rpim | 0.052 | 0.012 | 0.660 |
| Total number of observations | 115581 | 1173 | 8066 |
| Number of reflections | 30647 | ||
| <I/σ(I)> | 12 | 42.7 | 1.6 |
| Completeness [%] | 99.6 | 98.9 | 99 |
| Redundancy | 3.8 | 3.4 | 3.8 |
| CC(1/2) | 0.998 | 0.999 | 0.601 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 15% PEG-8000, 0.2 M magnesium chloride, 0.1 sodium cacodylate. A putative peptide ligand was added to the protein but not found during crystal structure analysis. |
