5J2N
HIV-1 reverse transcriptase in complex with DNA that has incorporated EFdA-MP at the P-(post-translocation) site and dTMP at the N-(pre-translocation) site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2015-10-02 |
| Detector | RDI CMOS_8M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 166.827, 169.885, 102.656 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.623 - 2.896 |
| R-factor | 0.2131 |
| Rwork | 0.212 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdbid 5J2M |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.443 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.12) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 53.620 | 53.620 | 3.040 |
| High resolution limit [Å] | 2.880 | 9.120 | 2.880 |
| Rmerge | 0.138 | 0.039 | 0.883 |
| Rmeas | 0.149 | 0.042 | 0.962 |
| Rpim | 0.055 | 0.016 | 0.376 |
| Total number of observations | 238525 | 7746 | 27685 |
| Number of reflections | 32900 | ||
| <I/σ(I)> | 13.4 | 41.2 | 2 |
| Completeness [%] | 99.0 | 99.6 | 93 |
| Redundancy | 7.2 | 6.8 | 6.2 |
| CC(1/2) | 0.997 | 0.999 | 0.669 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291.1 | 8% PEG4000, 25mM MES pH 6.0, 5mM magnesium sulfate |
