5J2I
Ternary complex crystal structure of DNA polymerase Beta with T:C mismatch at the primer terminus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-26 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.630, 80.150, 55.460 |
| Unit cell angles | 90.00, 107.51, 90.00 |
Refinement procedure
| Resolution | 25.113 - 2.400 |
| R-factor | 0.1909 |
| Rwork | 0.182 |
| R-free | 0.26850 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.976 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.072 | 0.408 |
| Number of reflections | 15747 | |
| <I/σ(I)> | 18.9 | |
| Completeness [%] | 94.7 | 91.4 |
| Redundancy | 2.4 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 16-18% PEG 3350, 50 mM Imidazole, 350 mM Sodium Acetate |
