5J2A
Ternary complex crystal structure of DNA polymerase Beta with A:C mismatch at the primer terminus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-08 |
Detector | RIGAKU SATURN 92 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.420, 79.220, 55.340 |
Unit cell angles | 90.00, 107.07, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.500 |
Rwork | 0.225 |
R-free | 0.29570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fms |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.114 | 0.564 |
Number of reflections | 14492 | |
<I/σ(I)> | 12.8 | 2 |
Completeness [%] | 99.7 | 99.8 |
Redundancy | 3.2 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 16-18% PEG 3350, 50 mM Imidazole, 350 mM Sodium Acetate |