5J28
Ki67-PP1g (protein phosphatase 1, gamma isoform) holoenzyme complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 61 |
| Unit cell lengths | 90.832, 90.832, 206.717 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.331 - 2.000 |
| R-factor | 0.1593 |
| Rwork | 0.154 |
| R-free | 0.19720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5inb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.861 |
| Data reduction software | XDS (Nov 3, 2014) |
| Data scaling software | Aimless (Nov 3, 2014) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.331 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.092 | 0.086 |
| Number of reflections | 64993 | |
| <I/σ(I)> | 20.5 | 3.1 |
| Completeness [%] | 100.0 | 92.2 |
| Redundancy | 10.4 | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 277 | 1.9 M Sodium Malonate pH 4.0 |
