5J1S

TorsinA-LULL1 complex, H. sapiens, bound to VHH-BS2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9778
Spacegroup name	P 21 21 21
Unit cell lengths	75.739, 90.659, 105.092
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.445 - 1.399
R-factor	0.144
Rwork	0.143
R-free	0.18790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4TVS_A
RMSD bond length	0.014
RMSD bond angle	1.250
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	61.445
High resolution limit [Å]	1.399
Number of reflections	132851
<I/σ(I)>	33
Completeness [%]	93.0
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	13 % PEG 6000 5 % MPD 100 mM MES, pH 6.5