5J1D

X-ray crystal structure of Phosphate binding protein (PBP) from Stenotrophomonas maltophilia

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-06-29
Detector	MARRESEARCH
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	37.780, 78.070, 56.380
Unit cell angles	90.00, 102.37, 90.00

Refinement procedure

Resolution	55.070 - 1.900
R-factor	0.1236
Rwork	0.122
R-free	0.15120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2q9t 2v3q 4f1v 4m1v
RMSD bond length	0.010
RMSD bond angle	1.309
Data reduction software	iMOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	55.071	39.035	2.000
High resolution limit [Å]	1.900	6.010	1.900
Rmerge		0.031	0.218
Number of reflections	25257
<I/σ(I)>	16.6	17.5	3.5
Completeness [%]	100.0	99.7	100
Redundancy	4.9	4.9	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.1M Bis-tris, pH 6.5, 20% PEG MME-5000