5J19
phospho-Pon binding-induced Plk1 dimerization
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.953 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 84.880, 91.997, 159.222 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.828 - 2.000 |
R-factor | 0.2142 |
Rwork | 0.213 |
R-free | 0.23560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1umw |
RMSD bond length | 0.009 |
RMSD bond angle | 1.161 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
Rmerge | 0.082 | 0.040 | 0.620 |
Total number of observations | 233958 | ||
Number of reflections | 42369 | ||
<I/σ(I)> | 12.7 | ||
Completeness [%] | 98.6 | 91.6 | 100 |
Redundancy | 5.5 | 5 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 5.5 | 289 | PEG 3350, NaCOOH |