5J0Z

Crystal structure of GLIC in complex with DHA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9787
Spacegroup name	C 1 2 1
Unit cell lengths	181.868, 133.320, 159.919
Unit cell angles	90.00, 102.36, 90.00

Refinement procedure

Resolution	30.000 - 3.250
R-factor	0.2331
Rwork	0.232
R-free	0.26110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hfi
RMSD bond length	0.013
RMSD bond angle	1.801
Data reduction software	iMOSFLM (7.2.1)
Data scaling software	Aimless (1.9.23)
Phasing software	PHASER (2.5.6)
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.310	3.340
High resolution limit [Å]	3.250	3.250
Number of reflections	64340
<I/σ(I)>	6.7
Completeness [%]	96.7	98.6
Redundancy	2.8	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	277	225 mM ammonium sulfate, 50 mM sodium acetate pH 4.0, 7.5-10% PEG 4000