5J02
Structure of the lariat form of a chimeric derivative of the Oceanobacillus iheyensis group II intron in the presence of NH4+, MG2+ and an inactive 5' exon.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.020, 100.600, 220.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.041 - 3.493 |
| R-factor | 0.2041 |
| Rwork | 0.203 |
| R-free | 0.22740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4faw |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.383 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.580 | |
| High resolution limit [Å] | 3.490 | 15.620 | 3.490 |
| Rmerge | 0.202 | 0.035 | 2.991 |
| Rmeas | 0.207 | 0.036 | 3.067 |
| Total number of observations | 548093 | ||
| Number of reflections | 24666 | 321 | 1737 |
| <I/σ(I)> | 12.32 | 60 | 1.2 |
| Completeness [%] | 99.7 | 96.4 | 96.2 |
| Redundancy | 22.2 | 20.4 | |
| CC(1/2) | 1.000 | 1.000 | 0.753 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 301.15 | 50 mM Na-Cacodylate pH 6.5, 225 mM NH4Cl, 115 mM MgCl2, 22% MPD |
