5J01
Structure of the lariat form of a chimeric derivative of the Oceanobacillus iheyensis group II intron in the presence of NH4+ and MG2+.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-12-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9786 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 100.930, 262.580, 69.040 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.755 - 3.390 |
R-factor | 0.2057 |
Rwork | 0.204 |
R-free | 0.23390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4faw |
RMSD bond length | 0.012 |
RMSD bond angle | 0.445 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.480 | |
High resolution limit [Å] | 3.390 | 15.150 | 3.390 |
Rmerge | 0.315 | 0.053 | 4.762 |
Number of reflections | 26308 | ||
<I/σ(I)> | 11.67 | 62.22 | 0.68 |
Completeness [%] | 99.7 | 95.8 | 96.9 |
Redundancy | 28.1 | 17.5 | |
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 301.15 | 50 mM Na-Cacodylate pH 6.5, 225 mM NH4Cl, 95 mM MgCl2, 22% MPD |